Synonyms

soluble Receptor Activator of NF-κB Ligand, TNFSF11, OPGL, ODF

TRANCE (TNF-related activation-induced cytokine)

Source

Expressed in E. coli.

Molecular Weight

Approximately 19.4kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Accession

O35235 :Pro143-Asp316

Biological Activity

Fully biologically active when compared to standard. Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells, The ED50 for this effect is 0.52 ng/ml.

Purity

> 96% by SDS-PAGE.

Endotoxin

＜0.01EU/μg rMusRANKL protein as determined by LAL method.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 300mM NaCl, pH7.4.

Reconstitution

Before use this product, please read the direction below carefully.

1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.