Product: Anti-Apaf-1 mAb, clone 2E12
Cat. No.: MC-287 (100 μg)
Background:
Programmed cell death (apoptosis) requires the
activation of caspases, which are in turn
regulated by adaptor proteins such as Apaf-1
(activator of apoptosis protease-activating
factor-1). The 136 kDa protein Apaf-1 is a
mammalian homolog of C. elegans/CED-4, and
is required for some pathways of cell death.
Apaf-1 promotes the aggregation and selfprocessing
of the initiator caspase, caspase-9 in
the presence of cytochrome c and dATP. It
consists of four function-determining regions: (1)
an N-terminal caspase activation and
recruitment domain (CARD), which is required
for binding to pro-caspase-9, (2) a central CED-
4 homologous region, (3) an Apaf-1 specific
region, and (4) 12 identified WD40 repeats at
the C-terminus of the protein.
Specificity:
Recognizes the CARD domain of human Apaf-1.
Species Reactivity:
Human, others not tested. Does not cross-react
with mouse or rat Apaf-1.
Ig Isotype:
Rat IgG2a
Immunogen:
FLAG-tagged recombinant human Apaf-1 (aa 1-
464) containing the N-terminal CARD and CED-
4 homologous domains.
Format:
100 μg of liquid Protein G purified monoclonal
antibody in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL.
Storage:
Store at 4°C short term. For long term, store at
-20°C. Avoid multiple freeze/thaws.
Applications and Suggested Dilutions:

Immunocytochemistry

Immunohistochemistry: Electron microscopy
on frozen sections.

Immunoprecipitation

Western blot

ELISA
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.