Product: Anti-BCMA, clone Vicky-1
Cat. No.: MC-558 (100 μg)
Synonyms:
B Cell Maturation Protein; TNFRSF 17; CD269
Specificity:
Recognizes human BCMA
Species Reactivity:
Human. Does not cross-react with mouse
BCMA. Other species not tested.
Isotype:
Rat IgG1
Immunogen:
Recombinant human BCMA:Fc. The extracellular
domain of human BCMA (aa 2-54) is
fused to the Fc portion of human IgG1.
Format:
100 μg of antibody at 1 mg/mL purified from
concentrated hybridoma tissue culture supernatant.
Provided in PBS with 0.02% sodium
azide.
Storage:
Store short term at 4°C and long term at -20°C.
Aliquot to avoid freeze/thaw cycles.
Applications:

Flow cytometry

Immunocytochemistry

Functional application: blocks binding of
BAFF to human BCMA
The researcher should determine the optimal
dilution for a specific application.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figure 1: Immunostaining of HEK 293 cells transfected with
a human BCMA expression plasmid (left panel), or mock
transfected (right panel).
Method: 3 days after transfection of cells with the indicated
constructs, cells were fixed with acetone or 4%
formaldehyde for 5 min. at room temperature. Slides were
blocked with normal IgG, and incubated for 1 hour with 5
μg/mL antibody to BCMA (human) (MC-558) in 1% BSA in 1
x PBS. After washes in PBS, samples were incubated with
the secondary antibody for 1 hour, washed in PBS and
revealed with StreptABComplex/HRP (Vector) and AEC.
Figure 2: Detection of endogenous human BCMA with
antibody to BCMA (MC-558).
Method: U266 cells (2x105) were incubated on ice for 30 min.
with 0.2 μg of antibody to BCMA (MC-558) or an isotype
control in 25 μL FACS buffer (PBS, 5% fetal calf serum,
0.02% azide). The primary antibody was revealed with
polyclonal antibody to Rat IgG (R-PE) and then analyzed by
flow cytometry.