Cat. No.: PC-510 (100 μg)
Synonyms:
Anti-Apoptosis Antagonizing Transcription
Factor, anti-Che-1
Background:
AATF was initially discovered as an interaction
partner of ZIP kinase (ZIPK), a member of
death-associated protein (DAP) kinase family of
pro-apoptotic serine/threonine kinases. AATF is
a phosphoprotein containing an acidic region
and a putative leucine zipper domain and
nuclear localization signal, features which are
typical of transcription factors. AATF inhibits the
ZIPK-mediated pro-apoptotic pathway and may
activate other anti-apoptotic pathways. Recently,
it has also been shown to protect neural cells
against oxidative damage induced by amyloid b-
peptide and to inhibit aberrant production of the
b-peptide by interacting with Par-4 (prostate
apoptosis response-4), another pro-apoptotic
leucine zipper protein that is associated with
neuronal degeneration in Alzheimer’s disease
(AD), suggesting that AATF may have potential
therapeutic applications in both familial and
sporadic forms of AD.
Specificity:
Recognizes AATF.
Positive Control:
Human small intestine cell lysate.
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
A 12 amino acid peptide from near the carboxyterminus
of human AATF (GenBank Accession
No. NP_036270).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5-1 μg/mL.

Immunohistochemistry: Use at ~10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of AATF in human
small intestine cell lysate with anti-AATF pAb at (A)
0.5 μg/mL, (B) 1 μg/mL, and (C) 2 μg/mL.
Figure 2: Immunohistochemistry of AATF in human
small intestine tissue with anti-AATFpAb at 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.