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Granulin / GRN Antibody Datasheet
 
 
 
 
 
 
 
 
 
Order or Inquire for GRN Antibody product
 
 
 
 
 
 
 
Detection limit is 4 ng/lane in WB
 
 
 
 
 
 
Granulin / GRN抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human Granulin protein ( Catalog#10826-H08H )
 
 
 
 
抗体类型: 
 
Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
 
 
 
 
克隆号 :
 
 
 
10C9E2
 
 
 
 
抗体宿主 :
 
 
Mouse  IgG2b
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Granulin / GRN ( rhGRN; Catalog#10826-H08H; Met 1-Leu 593; NP_002078.1 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
Granulin / GRN抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human Granulin / GRN
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot :
 
This antibody can be used at 1-2μg/mL with the appropriate secondary reagents to detect GRN in WB. Using a DAB detection system, the detection limit for GRN is approximately 4 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.Avoid repeated freeze-thaw cycles.
 
 
 
 
Granulin / GRN Antibody Related Products & Topics
 
 
Granulin / GRN Antibody 相关研究领域:
 
Cancer>>Growth Factor>>Granulin/Epithelin Family>>Granulin/Progranulin/GRN
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Granulin/Progranulin
 
Human
 
Granulin/Progranulin Protein, Recombinant 
 
10826-H08H
 
 
 
Granulin/Progranulin
 
Mouse
 
Granulin/Progranulin Protein, Recombinant 
 
50396-M08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human Progranulin/PGRN
 
WB
 
Mouse Monoclonal Antibody
 
10826-MM01
 
 
 
Human Progranulin/PGRN
 
ELISA
 
Mouse Monoclonal Antibody
 
10826-MM02
 
 
 
Human Progranulin/PGRN
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
10826-RP03
 
 
 
Human Progranulin/PGRN
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10826-RP04
 
 
 
 
 
 Granulin / GRN抗体背景综述
 
 
Granulin (GRN), also known as PC cell derived growth factor (PCDGF), is a ubiquitously expressed glycosylated protein which contains seven tandem repeats (55-57 aa consensus) of a highly conserved 12-cysteine granulin/epithelin motif. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Both the peptides and intact granulin protein regulate cell growth. Different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth, and thus perform important functions in normal development, wound healing, and tumorigenesis. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD), an autosomal dominant neurodegenerative.
 
 
参考文献
 
 
 
Bateman, A. et al.,1991, Biochem. Biophys. Res. Commun. 173(3): 1161-8.
 
Hoque, M. et al., 2003, Mol. Cell. Biol.  23 (5): 1688 -1702. 
 
Hoque, M. et al., 2005, J Biol Chem. 280 (14):13648-57.
 
Zhou, Ying. et al., 2008, Cell. Signal.  20 (5): 942-8.
 
 
 
 
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