Product: Anti-CARMA1 pAb
Cat. No.: PC-482 (100 μL)
Synonyms:
CARD11; Caspase Recruitment Domaincontaining
Protein 11; Bimp3; CARD-containing
MAGUK Protein 1.
Specificity:
Recognizes CARMA1. Detects a band of ~130
kDa by Western blot.
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
N-terminal human CARMA1 (aa 1-221
containing the CARD domain).
Format:
100 μL of liquid polyclonal antibody with 0.02%
sodium azide.
Storage:
Store at 4°C short term, -20°C long term. Aliquot
to avoid repeated freeze/thaw cycles.
Applications:

Western blot

Immunocytochemistry

Immunohistochemistry
The researcher should determine the optimal
dilution for a specific application.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figure 1: Western blot analysis of CARMA1 by the antihuman
CARMA1 antibody.
Method: Jurkat T cells (8 x 106) were activated with CD3
monoclonal antibody (TR66, 10 μg/mL) and protein
distribution of lipid raft (R) or non-raft (S) fractions was
analyzed by detergent fractionation and immunoblotting
(1:2,000) with anti-CARMA1 in nontransfected Jurkat T cells.
Figure 2: Immunohistochemistry analysis of CARMA1 by the
anti-human CARMA1 antibody.
Method: Human T cells (clone 6396p5.1.2) were incubated
with CD3 monoclonal antibody for 20 min on ice, followed by
incubation with secondary goat anti-mouse for 15 min at 4°C
or 37°C. Cells were stained for CD3 (green),
phosphotyrosine (red) and CARMA1 antibody (1:100 dilution;
blue) and analyzed by confocal microscopy